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human il 7 duoset elisa development system  (R&D Systems)


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    R&D Systems human il 7 duoset elisa development system
    Figure 2. MVA-hIL-7-Fc induces the production of functional hIL-7-Fc. (a) Quantification of hIL7-Fc in serum was performed over time after MVA-hIL-7-Fc injection using a specific <t>ELISA.</t> (b) Expression of IL-7 receptor (CD127) at the surface of T cells (percentage of CD3+ cells) and (c) the level of STAT 5 phosphorylation (mean fluorescence intensity (MFI) were evaluated in CD3+ T cells by flow cytometry. Each colored line represents one animal.
    Human Il 7 Duoset Elisa Development System, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+il+7+duoset+elisa+development+system/Human+IL-7+DuoSet+ELISA/pm36315906-57-11-18
    Average 94 stars, based on 22 article reviews
    human il 7 duoset elisa development system - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Intravenous injection of a novel viral immunotherapy encoding human interleukin-7 in nonhuman primates is safe and increases absolute lymphocyte count."

    Article Title: Intravenous injection of a novel viral immunotherapy encoding human interleukin-7 in nonhuman primates is safe and increases absolute lymphocyte count.

    Journal: Human vaccines & immunotherapeutics

    doi: 10.1080/21645515.2022.2133914

    Figure 2. MVA-hIL-7-Fc induces the production of functional hIL-7-Fc. (a) Quantification of hIL7-Fc in serum was performed over time after MVA-hIL-7-Fc injection using a specific ELISA. (b) Expression of IL-7 receptor (CD127) at the surface of T cells (percentage of CD3+ cells) and (c) the level of STAT 5 phosphorylation (mean fluorescence intensity (MFI) were evaluated in CD3+ T cells by flow cytometry. Each colored line represents one animal.
    Figure Legend Snippet: Figure 2. MVA-hIL-7-Fc induces the production of functional hIL-7-Fc. (a) Quantification of hIL7-Fc in serum was performed over time after MVA-hIL-7-Fc injection using a specific ELISA. (b) Expression of IL-7 receptor (CD127) at the surface of T cells (percentage of CD3+ cells) and (c) the level of STAT 5 phosphorylation (mean fluorescence intensity (MFI) were evaluated in CD3+ T cells by flow cytometry. Each colored line represents one animal.

    Techniques Used: Functional Assay, Injection, Enzyme-linked Immunosorbent Assay, Expressing, Phospho-proteomics, Fluorescence, Flow Cytometry

    Related Articles

    Produced:

    Article Title: A novel virotherapy encoding human interleukin-7 improves ex vivo T lymphocyte functions in immunosuppressed patients with septic shock and critically ill COVID-19
    Article Snippet: .. The hIL-7-Fc produced in supernatants of infected cells was quantified using the human IL-7 DuoSet ELISA Development System from R&D Systems (Minneapolis, Minnesota) according to the manufacturer's instructions. .. The IL-7 concentration of each sample was calculated using a standard curve established with the IL-7 standard provided by R&D Systems.

    Infection:

    Article Title: A novel virotherapy encoding human interleukin-7 improves ex vivo T lymphocyte functions in immunosuppressed patients with septic shock and critically ill COVID-19
    Article Snippet: .. The hIL-7-Fc produced in supernatants of infected cells was quantified using the human IL-7 DuoSet ELISA Development System from R&D Systems (Minneapolis, Minnesota) according to the manufacturer's instructions. .. The IL-7 concentration of each sample was calculated using a standard curve established with the IL-7 standard provided by R&D Systems.

    Enzyme-linked Immunosorbent Assay:

    Article Title: A novel virotherapy encoding human interleukin-7 improves ex vivo T lymphocyte functions in immunosuppressed patients with septic shock and critically ill COVID-19
    Article Snippet: .. The hIL-7-Fc produced in supernatants of infected cells was quantified using the human IL-7 DuoSet ELISA Development System from R&D Systems (Minneapolis, Minnesota) according to the manufacturer's instructions. .. The IL-7 concentration of each sample was calculated using a standard curve established with the IL-7 standard provided by R&D Systems.

    Article Title: Intravenous injection of a novel viral immunotherapy encoding human interleukin-7 in nonhuman primates is safe and increases absolute lymphocyte count
    Article Snippet: .. The concentration of hIL-7-Fc in frozen sera was determined using the human IL-7 DuoSet® ELISA Development System from R&D Systems according to the provider’s instructions. .. The hIL-7-Fc concentration of each sample was calculated using a standard curve established with a recombinant hIL-7-Fc protein, the exact counterpart of the MVA-expressed hIL-7-Fc (produced on CHO cells by Geneart (Regensburg, Germany)).

    Article Title: Intravenous injection of a novel viral immunotherapy encoding human interleukin-7 in nonhuman primates is safe and increases absolute lymphocyte count.
    Article Snippet: .. The concentration of hIL7-Fc in frozen sera was determined using the human IL-7 DuoSet® ELISA Development System from R&D Systems according to the provider’s instructions. .. The hIL-7-Fc concentration of each sample was calculated using a standard curve established with a recombinant hIL-7-Fc protein, the exact counterpart of the MVA-expressed hIL-7-Fc (produced on CHO cells by Geneart (Regensburg, Germany)).28 The area under the serum concentration vs. time curve (AUC) was calculated with Prism 9.0 software (GraphPad Software, LLC) using the trapezoid rules.

    Concentration Assay:

    Article Title: Intravenous injection of a novel viral immunotherapy encoding human interleukin-7 in nonhuman primates is safe and increases absolute lymphocyte count
    Article Snippet: .. The concentration of hIL-7-Fc in frozen sera was determined using the human IL-7 DuoSet® ELISA Development System from R&D Systems according to the provider’s instructions. .. The hIL-7-Fc concentration of each sample was calculated using a standard curve established with a recombinant hIL-7-Fc protein, the exact counterpart of the MVA-expressed hIL-7-Fc (produced on CHO cells by Geneart (Regensburg, Germany)).

    Article Title: Intravenous injection of a novel viral immunotherapy encoding human interleukin-7 in nonhuman primates is safe and increases absolute lymphocyte count.
    Article Snippet: .. The concentration of hIL7-Fc in frozen sera was determined using the human IL-7 DuoSet® ELISA Development System from R&D Systems according to the provider’s instructions. .. The hIL-7-Fc concentration of each sample was calculated using a standard curve established with a recombinant hIL-7-Fc protein, the exact counterpart of the MVA-expressed hIL-7-Fc (produced on CHO cells by Geneart (Regensburg, Germany)).28 The area under the serum concentration vs. time curve (AUC) was calculated with Prism 9.0 software (GraphPad Software, LLC) using the trapezoid rules.



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    Figure 2. MVA-hIL-7-Fc induces the production of functional hIL-7-Fc. (a) Quantification of hIL7-Fc in serum was performed over time after MVA-hIL-7-Fc injection using a specific <t>ELISA.</t> (b) Expression of IL-7 receptor (CD127) at the surface of T cells (percentage of CD3+ cells) and (c) the level of STAT 5 phosphorylation (mean fluorescence intensity (MFI) were evaluated in CD3+ T cells by flow cytometry. Each colored line represents one animal.
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    Figure 2. MVA-hIL-7-Fc induces the production of functional hIL-7-Fc. (a) Quantification of hIL7-Fc in serum was performed over time after MVA-hIL-7-Fc injection using a specific <t>ELISA.</t> (b) Expression of IL-7 receptor (CD127) at the surface of T cells (percentage of CD3+ cells) and (c) the level of STAT 5 phosphorylation (mean fluorescence intensity (MFI) were evaluated in CD3+ T cells by flow cytometry. Each colored line represents one animal.
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    Image Search Results


    Figure 2. MVA-hIL-7-Fc induces the production of functional hIL-7-Fc. (a) Quantification of hIL7-Fc in serum was performed over time after MVA-hIL-7-Fc injection using a specific ELISA. (b) Expression of IL-7 receptor (CD127) at the surface of T cells (percentage of CD3+ cells) and (c) the level of STAT 5 phosphorylation (mean fluorescence intensity (MFI) were evaluated in CD3+ T cells by flow cytometry. Each colored line represents one animal.

    Journal: Human vaccines & immunotherapeutics

    Article Title: Intravenous injection of a novel viral immunotherapy encoding human interleukin-7 in nonhuman primates is safe and increases absolute lymphocyte count.

    doi: 10.1080/21645515.2022.2133914

    Figure Lengend Snippet: Figure 2. MVA-hIL-7-Fc induces the production of functional hIL-7-Fc. (a) Quantification of hIL7-Fc in serum was performed over time after MVA-hIL-7-Fc injection using a specific ELISA. (b) Expression of IL-7 receptor (CD127) at the surface of T cells (percentage of CD3+ cells) and (c) the level of STAT 5 phosphorylation (mean fluorescence intensity (MFI) were evaluated in CD3+ T cells by flow cytometry. Each colored line represents one animal.

    Article Snippet: The concentration of hIL7-Fc in frozen sera was determined using the human IL-7 DuoSet® ELISA Development System from R&D Systems according to the provider’s instructions.

    Techniques: Functional Assay, Injection, Enzyme-linked Immunosorbent Assay, Expressing, Phospho-proteomics, Fluorescence, Flow Cytometry